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As of March 3rd of 2025, the BSC Assay laboratory changed the BSC Certification requirements for Azure B. Test Current Requirement Old Requirement UV-Vis 645-649 nm 1.02-1.18 R ratio Quantitation Minimum 80% (Updated from a Minimum 89%) HPLC Thiazine Components Azure B Minimum 70% ( Updated from a “Majority of Azure B”) Blood Smear stain […]
Richard W. Dapson writes, on 17th May 2021: Carminic acid and its aluminum lake, carmine, are important histological dyes, but commercial trade in these products has undergone massive shifts in the last few decades due to market forces way beyond histology. Originally a textile dye from Central and South America, indigenous people harvested a parasitic […]
Are fluorescent probes biological stains? On 29th April 2021 Richard W. Horobin wrote: There are various communities of clinical lab workers who use “traditional” microscopic staining methods, such as hematoxylin and eosin, and the Gram or Papanicolaou stains. However even these three stains are most commonly used by three quite distinct groups of people, typically […]
In December 2018 the Biological Stain Commission published Version 1.0 of an online glossary of words, names and short phrases relating to staining and histotechnique, including fixation, tissue processing and, of course, staining – with dyes, histochemical methods and immunohistochemistry. Notable features are explanations of physical, chemical and immunological terms used in explanations of what […]
Certified dyes [Updated October 2022] The following additions and changes have been made since 2002, the year of publication of the 10th edition of Conn’s Biological Stains and of a detailed account of tests then used in the Commission’s assay laboratory (Penney et al. 2002). The following notes (alphabetically by names of dyes) refer to […]