Conn’s Biological Stains

Conn’s Biological Stains [10th edition]


For 80 years Conn’s Biological Stains has been a standard reference for all who use colorants and fluorochromes in the biological and medical sciences. This long awaited tenth edition appears 25 years after R.D. Lillie’s ninth and has been completely rewritten to reflect the increase in range of uses. Although the staining of microscopical preparations continues to expand, the uses of dyes and fluorochromes now extend far beyond this traditional application. This book includes the first critical overview of the whole range of low molecular weight fluorescent probes, outside the catalogue literature. The first eight chapters are essays, by leading experts, on important aspects of colorants and their uses. Most of the remainder of the book consists of descriptions by Dr Horobin of the properties and recent applications of hundreds of individual compounds, in about twenty chemical classes. The last chapter reviews the procedures employed at the Biological Stain Commission’s laboratory to assay and test dyes and certify them as suitable for their intended applications. The book concludes with a 111-page Bibliography that gives complete citations (all authors, titles, complete journal names, page ranges etc) for references in all the chapters, and an Index of dye names and other topics.

Conn’s Biological Stains is published on behalf of the Biological Stain Commission, a non-profit corporation that exists to promote the availability of effective dyes and stains, through testing, certification, research, publication and education.


Ch 1. Introduction to dyes and stains (F.H. Kasten)
Ch 2. The history of staining (B. Bracegirdle)
Ch 3. Nomenclature and classification of dyes and other coloring agents (J.A. Kiernan)
Ch 4. Applications of dyes, fluorochromes and pigments (M. Wainwright)
Ch 5. Mechanisms of biological staining (R.W. Horobin)
Ch 6. Dye purity and dye standardisation for biological staining (H. Lyon)
Ch 7. Reactive staining reagents and fluorescent labels (J.C. Stockert)
Ch 8. The use of dyes and fluorochromes as indicators (M. Wainwright)
Ch 9. Nitroso and nitro dyes (R.W. Horobin)
Ch 10. Monoazo dyes (R.W. Horobin)
Ch 11. Dis-, tris- and poly azo dyes (R.W. Horobin)
Ch 12. Diazonium salts and their reaction products with coupling agents (R.W. Horobin)
Ch 13. Tetrazolium salts and formazans (R.W. Horobin)
Ch 14. Amino di- and triarylmethane dyes (R.W. Horobin)
Ch 15. Hydroxy triarylmethanes (R.W. Horobin)
Ch 16. Xanthenes (R.W. Horobin)
Ch 17. Acridines and phenanthridines (R.W. Horobin)
Ch 18. Azines (R.W. Horobin)
Ch 19. Oxazines and related dyes (R.W. Horobin)
Ch 20. Thiazines (R.W. Horobin)
Ch 21. Romanowsky-Giemsa stains (D.H. Wittekind)
Ch 22. Polyene dyes and fluorochromes (R.W. Horobin)
Ch 23. Polymethines – 1. Cyanines, oxonols, benzimidazoles, indolenines and azamethines (R.W. Horobin)
Ch 24. Polymethine dyes – 2. Styryls, thiazoles, coumarins and flavonoids (R.W. Horobin)
Ch 25. Carbonyl dyes including indigoids, anthraquinones and naphthalimides (R.W. Horobin)
Ch 26. Phthalocyanines, porphyrins and related aza[18]annulenes (R.W. Horobin)
Ch 27. Miscellaneous inorganic and organic substances used as biological stains (J.A. Kiernan and R.W. Horobin)
Ch 28. Methods for testing biological stains (D.P. Penney and J.M. Powers with the assistance of C. Willis, M. Frank and C. Churukian)

ISBN 9781859960998

BIOS Scientific Publishers (2002)

CRC Press (Taylor & Francis Group), an Informa business.
Informa plc Registered Office
Mortimer House, 37-41 Mortimer Street
London W1T 3JH
United Kingdom

Check availability and compare prices